Sodium valproate enhances urethane tumorigenicity in lungs of male but not female mice

Donatas Stakišaitis, S. Uleckiene, Janina Didžiapetrienė, Angelija Valančiūtė, Paulius Matusevicius

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    Abstract

    In the study, the possible effect of sodium valproate (NaVP) on urethane-induced lung tumors in mice has been evaluated. BALB/c mice (n = 120; 4–6 weeks old, both sexes) were used in the following groups: 1) urethane-treated, 2) urethane–NaVP-treated, 3) only NaVP-treated, 4) control. In the same groups, castrated male mice (n = 48) were investigated. Urethane was given by intraperitoneal injections 10 mg/mouse, twice a week, the total dose 50 mg/mouse. In NaVP-treated mice, the 0.4 % NaVP aqueous solution was offered to mice ad libitum. The duration of the experiment was 6 months. The number of tumors per mouse in urethane– NaVP-treated males was significantly higher than in those treated with urethane only (13.82 ± 1.12 vs 6.77 ± 0.43, p < 0.0001). No significant difference in the number of tumors per mouse was revealed while comparing the female urethane- and urethane–NaVP-treated groups (6.50 ± 0.79 vs 8.15 ± 0.55, p = 0.105). No difference in the number of tumors per mouse was found in urethane–NaVP-treated castrated males as compared with urethane-treated castrated males. However, in the urethane–NaVP-treated castrated males the number of tumors per mouse was significantly lower than in analogous non-castrated males (7.8 ± 1.67 vs 13.82 ± 1.12, p < 0.01). NaVP combined with urethane potentiates urethane tumorigenicity in BALB/c non-castrated but not in female and castrated male mice. These data indicate an important role of testosterone in the urethane-NaVP induced lung tumorigenesis.
    Original languageEnglish
    Pages (from-to)667-678
    JournalEXCLI Journal
    Volume13
    Publication statusPublished - 2014

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    Keywords

    • Sodium valproate
    • Urethane
    • Mice

    Cite this

    Stakišaitis, D., Uleckiene, S., Didžiapetrienė, J., Valančiūtė, A., & Matusevicius, P. (2014). Sodium valproate enhances urethane tumorigenicity in lungs of male but not female mice. EXCLI Journal, 13, 667-678.